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This No. face is a rage comic character that is used to dismiss or disapprove of another person’s idea. When in a comment, it is sometimes used as a standalone reaction image in a similar context to other negative rage faces like 'Are You Serious' face, the 'Yao Ming' face as well as the 'Are You F**king Kidding Me' face.
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How do I upload a custom emoji to Slack?

To upload your custom emoji to Slack workspace follow these steps.
Click the arrow beside your workspace name and select "customize slack".

Click "add custom emoji" and select your emoji.
Choose a name and click save. The emoji should now be available for use in your Slack workspace!